Optimization of High Grade Glioma Cell Culture from Surgical - JoVE

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The fraction of tumour cells expressing CD133 (Prominin-1), a marker for both neural stem cells and brain cancer stem cells3,4,5,6, is enriched after radiation in gliomas. cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction of tumour Notch inhibition with GSIs did not alter the DNA damage response of glioma stem cells following radiation, but rather impaired radiation-induced Akt activation and upregulated levels of the truncated apoptotic isoform of Mcl-1 (Mcl-1s). Taken together, our results suggest a critical role of Notch to promote radioresistance of glioma stem cells.

Glioma stem cells promote radioresistance by preferential activation of the dna damage response

We have examined DNA repair in five stem and nonstem glioma cell lines. The population doubling time was … Comparative analysis of DNA repair in stem and nonstem glioma cell cultures. It has been reported that cancer stem cells may contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. We have examined DNA repair in five stem and nonstem glioma cell lines. Cancer stem cells contribute to glioma radioresistance by an increase of DNA repair capacity through preferential activation of the DNA damage response checkpoints. Potential therapies that modulate or target cancer stem cells are also reviewed. cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity.

In both cell culture and the brains of immunocompromised mice, CD133-expressing glioma cells survive ionizing radiation in increased proportions Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction of tumour cells expressing CD133 (Prominin-1), a marker for both neural stem cells and brain cancer stem cells, is enriched after radiation in gliomas. Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity.

Optimization of High Grade Glioma Cell Culture from Surgical - JoVE

Download Citation | On Jan 1, 2008, R.J. Arceci published Glioma stem cells promote radioresistance by preferential activation of the DNA damage response | Find, read and cite all the research you The mechanisms underlying tumour radioresistance have remained elusive. Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage The blue social bookmark and publication sharing system. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response.

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Within these tumors, a CD133-positive cancer-initiating neural stem cell (NSC) population was shown to be resistant to gamma radiation through preferential activation of the DNA double-strand break (DSB) response machinery, including the ataxia-telangiectasia-mutated (ATM) kinase. Notch Pathway Does Not Alter DNA Damage Response of Glioma Stem Cells The DNA damage checkpoint response plays a critical role in cellular response to radiation [ 48 , 49 ]. Our previous study showed that increased activation of the DNA damage response is implicated in radioresistance of the glioma stem cells [ 7 ]. Neoplastic Stem Cells Subject Areas on Research 4-Hydroperoxycyclophosphamide purging of breast cancer from the mononuclear cell fraction of bone marrow in patients receiving high-dose chemotherapy and autologous marrow support: a phase I trial. McLendon et al., "Glioma stem cells promote radioresistance by preferential activation of the DNA damage response," Nature, vol.

Glioma stem cells promote radioresistance by preferential activation of the dna damage response

Nature. 2006;444:756–60.
Bilismens utveckling

Man et al. now show that GSCs have co-opted a neurodevelopmental program to activate Rac1 to promote defining features of GSCs.

Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity.
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Optimization of High Grade Glioma Cell Culture from Surgical - JoVE

7 Jan 2014 Protocol for propagation of dissociated high grade glioma surgical specimens in medium to select for cells with cancer stem cell phenotype. Mario Capecchi describes the challenges in developing the technique of gene targeting, which allows the manipulation of genes from tissue culture cells to 8 Oct 2007 Our DNA is packaged in chromosomes, which occur in pairs – one Smithies initially tried to repair mutated genes in human cells.